Ultrasensitive endonuclease activity and inhibition detection using gold nanoparticle-enhanced fluorescence polarization.

نویسندگان

  • Yong Huang
  • Shulin Zhao
  • Zhen-Feng Chen
  • Yan-Cheng Liu
  • Hong Liang
چکیده

We have developed a highly sensitive and selective fluorescence polarization assay method based on the specificity of the DNA cleavage reaction with the enhancement of gold nanoparticles (AuNPs) for assaying endonuclease activity and inhibition. This assay can detect EcoRI endonuclease down to 5.0×10(-4) U mL(-1) with a detection range from 5.0×10(-4) to 10 U mL(-1).

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عنوان ژورنال:
  • Chemical communications

دوره 47 16  شماره 

صفحات  -

تاریخ انتشار 2011